Use of synthetic antigens to determine the epitope specificities of monoclonal antibodies against the 3-deoxy-D-manno-octulosonate region of bacterial lipopolysaccharide

L Brade, P Kosma, B J Appelmelk, H Paulsen, H Brade
1987 Infection and Immunity  
Mouse monoclonal antibodies were raised against heat-killed bacteria of the Re mutant R595 of Salmonella minnesota and characterized by the passive hemolysis and passive hemolysis inhibition tests and by double immunodiffusion experiments using lipopolysaccharide (LPS) from different rough mutants of S. minnesota and synthetic antigens. The latter were copolymerization products of acrylamide with the a-and . Brade, H., C. Galanos, and 0. Luderitz. 1983. Isolation of 3-deoxy-D-mannooctulosonic
more » ... -mannooctulosonic acid disaccharide from Salmonella minnesota rough-form lipopolysaccharides. Eur. J. Biochem. 131:201-203. 4. Brade, H., H. Moll, and E. T. Rietschel. 1985. Structural investigations on the inner core region of lipopolysaccharides from Salmonella minnesota rough mutants. Biomed. Mass Spectrom. 12:602-609.
doi:10.1128/iai.55.2.462-466.1987 fatcat:h2o4qti73zh6hkntpwqr63dy4m