Sequence Analysis of Chloroplast chlB Gene of Medicinal Ephedra Species and Its Application to Authentication of Ephedra Herb

Yahong Guo, Ayako Tsuruga, Shigeharu Yamaguchi, Koji Oba, Kasumi Iwai, Setsuko Sekita, Hajime Mizukami
2006 Biological and Pharmaceutical Bulletin  
Chloroplast chlB gene encoding subunit B of light-independent protochlorophyllide reductase was amplified from herbarium and crude drug specimens of Ephedra sinica, E. intermedia, E. equisetina, and E. przewalskii. Sequence comparison of the chlB gene indicated that all the E. sinica specimens have the same sequence type (Type S) distinctive from other species, while there are two sequence types (Type E1 and Type E2) in E. equisetina. E. intermedia and E. prezewalskii revealed an identical
more » ... d an identical sequence type (Type IP). E. sinica was also identified by digesting the chlB fragment with Bcl I. A novel method for DNA authentication of Ephedra Herb based on the sequences of the chloroplast chlB gene and internal transcribed spacer of nuclear rRNA genes was developed and successfully applied for identification of the crude drugs obtained in the Chinese market.
doi:10.1248/bpb.29.1207 pmid:16755018 fatcat:2aqb7xvqzndi5ctvl43ne5ialq