Urban trees and forests provide multiple ecosystem services (ES), including temperature regulation, carbon sequestration, and biodiversity. Interest in ES has increased amongst policymakers, scientists, and citizens given the extent and growth of urbanized areas globally. However, the methods and techniques used to properly assess biodiversity and ES provided by vegetation in urban environments, at large scales, are insufficient. Individual tree identification and characterization are some of

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... e most critical issues used to evaluate urban biodiversity and ES, given the complex spatial distribution of vegetation in urban areas and the scarcity or complete lack of systematized urban tree inventories at large scales, e.g., at the regional or national levels. This often limits our knowledge on their contributions toward shaping biodiversity and ES in urban areas worldwide. This paper provides an analysis of the state-of-the-art studies and was carried out based on a systematic review of 48 scientific papers published during the last five years (2016–2020), related to urban tree and greenery characterization, remote sensing techniques for tree identification, processing methods, and data analysis to classify and segment trees. In particular, we focused on urban tree and forest characterization using remotely sensed data and identified frontiers in scientific knowledge that may be expanded with new developments in the near future. We found advantages and limitations associated with both data sources and processing methods, from which we drew recommendations for further development of tree inventory and characterization in urban forestry science. Finally, a critical discussion on the current state of the methods, as well as on the challenges and directions for future research, is presented.