Preparation of Autofluorescent Polyacrylamide Nanoparticles

Qiao Wang, Jinke Wang
2018 Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)   unpublished
Nowadays, tumor imaging is widely used in biomedical field. However, the materials of imaging is rarely and strict. This study developed a method for preparing autofluorescent polyacrylamide nanoparticles (NPs). The polyacrylamide NPs were treated with glutaraldehyde, and then removed excess glutaraldehyde through ultrafiltration, glycine neutralization and ethanol precipitation, respectively. Based on this method, the polyacrylamide NPs showed strong nearinfrared fluorescence (NIRF) at the
more » ... sion wavelength of both 720 and 820 nm. Additionally, the nanoparticles were efficiently internalized into RAW264.7 cells and displayed strong green and red fluorescence observed with fluorescence microscope. Therefore, the NPs are expected to be applied to tumor imaging in future.
doi:10.2991/icammce-18.2018.96 fatcat:fvcvnymtrzbndn26vba3ii543e