Indonesian tobacco is largely used for cigarettes. The Indonesian cigarettes export market is currently affected because many countries have signed the Framework Convention on Tobacco Control (FCTC) which is under the auspices of the World Health Organization (WHO). Exploration of the potential of tobacco as a raw material of a hygienic product is an alternative solution for the sustainability of tobacco cultivation while increasing the empowerment of tobacco farmers. The high nicotine content

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... f tobacco is needed to be used as raw material of a hygienic product. The purpose of this research is to isolate nicotine from tobacco leaves and find out the tobacco with the highest nicotine content. The design of this study is experimental laboratory. Tobacco leaf samples were taken from tobacco producing regions in Indonesia. Nicotine was isolated from tobacco leaves using ethanol 96%. Quantitative analysis of nicotine compound was carried out using Gas Chromatography Mass Spectroscopy (GCMS). The result showed nicotine content in tobacco leaf samples ranged 0.08-2.53% and the highest nicotine content is in tobacco samples from Purwodadi.