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Enhancing SDS-PAGE Detection of Dilute Extracellular Polystyrene Degrading Enzymes Expressed by Bacillus megaterium Strain via Centrifugal Freeze Concentration Method
Trends in Sciences
This study aims to enhance the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) detection and visualization of extracellular polystyrene degrading enzyme expressed by Bacillus megaterium as a result of utilizing polystyrene as a sole carbon source. Dilute polystyrene degrading enzyme expressed in the culture supernatant could not be directly visualized from SDS-PAGE stained with colloidal Coomassie blue stain as it falls below its sensitivity or detection limit level. Todoi:10.48048/tis.2022.5613 fatcat:y2j5rzuu3ba3raju4zudyzlany