A RATIONAL SYNTHESIS OF MAGNETIC NANOPARTICLES INCORPORATED HORSERADISH PEROXIDASE NANOFLOWER AND ITS USE FOR THE REMOVAL OF PHENOL THROUGH OXIDATIVE COUPLING REACTION WITH GREAT REUSABILITY
Mugla Journal of Science and Technology
Herein, we report preparation of magnetic hybrid nanoflower (MhNF) formed of horseradish peroxidase (HRP), copper ions (Cu 2+ ) and iron oxide nanoparticles (Fe3O4 NPs) with greatly enhanced activity, stability, reusability and applicability for use in removal of phenol from aqueous solution. In this study, pre-synthesized small sized Fe3O4 NPs were immobilized to synthesized HRP-Cu 2+ hybrid nanoflower (hNFs). The synthesized MhNFs were characterized using SEM, EDX and XRD analysis. We also
... racterized the chemical structures of free HRP and MhNF using FTIR. The protein encapsulation yields of synthesized MhNFs were determined spectrophotometrically and calculated as ~98% for all synthesis conditions. The activities of free HRP, HRP-based non-magnetic hybrid nanoflower (hNF) and MhNF were calculated as 5847.6, 16738.2, and 18830.5 EU/mg, respectively. The optimum pH of the synthesized MhNFs for phenol removal was determined as pH 7. MhNF was easily and rapidly separated from reaction medium by an external magnetic field to be re-used. Even after seven cycles, MhNFs maintained more than 75% of their initial activity. We believe that the repeated use can reduce the capital and operation costs. The results are very promising in terms of increasing efficiency and applicability of HRP in industrial application especially for wastewater treatment.