Successful development of bacteriocins into therapeutic formulation for treatment of MRSA-skin infection in a murine model

Kirill V Ovchinnikov, Christian Kranjec, Tage Thorstensen, Harald Carlsen, Dzung B Diep
2020 Antimicrobial Agents and Chemotherapy  
The emergence of antibiotic resistant pathogens has caused a serious world-wide problem in infection treatments in recent years. One of these pathogens is the methicillin-resistant Staphylococcus aureus (MRSA) which is a major cause of skin and soft tissue infections. Alternative strategies and novel sources of antimicrobials to solve antibiotic resistance problems are urgently needed. In this study, we explored the potential of two broad-spectrum bacteriocins, garvicin KS and micrococcin P1,
more » ... d micrococcin P1, in skin infection treatments. These two bacteriocins were acting synergistically with each other and with penicillin G in killing MRSA in vitro The minimum inhibitory concentration (MIC) of the antimicrobials in the three-component mixture was 40 ng/ml for micrococcin P1 and 2 μg/ml for garvicin KS and penicillin G, which were 62, 16 and at least 1250 times less when compared to their MICs when assessed individually. To assess its therapeutic potential further, we challenged this three-component formulation in a murine skin infection model with the multidrug-resistant luciferase-tagged MRSA Xen31, a strain derived from the clinical isolate S. aureus ATCC 33591. Using the tagged luciferase activity as a reporter for the presence of Xen31 in wounds, we evidently demonstrated that the three-component formulation was efficient to eradicate the pathogen from treated wounds. Furthermore, compared to Fucidin cream which is an antibiotic commonly used in skin infection treatments, our formulation was also superior in terms of preventing resistance development.
doi:10.1128/aac.00829-20 pmid:32958719 fatcat:jpzind6dofdvlir36igh75umkq