The purpose of presented study was determination of the impact of selected phys-icochemical parameters, strongly influencing the soil biological life: water potential (pF), oxygen availability for microorganisms (ODR), redox potential (Eh), content of Mg, Ca and total organic carbon (TOC) on soil DNA concentration. Undisturbed loess soil material was taken to metal cylinders (100 cm 3) from four depths (0-20; 20-40; 40-60 and 60-80 cm), what make possibility for description of DNA content and

more »

... s distribution in the whole soil profile. Our results revealed significant (p<0.05) positive relationships between soil DNA content and measured values of ODR (r = 0.94***), Eh (r = 0.52**) and TOC (r = 0.98***), what were confirmed by high values of correlation coefficients (r). Whereas, significant negative interrelationships between soil DNA and pF (r =-0.57*) or Mg content (r =-0.79***) were determined. However, in the current experiment conditions , we did not found significant correlation between Ca presence and DNA content (p>0.05). Significant (p<0.05) decrease of DNA concentration by 62.8% with an increase of soil depth was noted, what was undoubtedly connected with spatial distribution of microorganisms in the soil profile and its likes for surface layers colonization.