Effect of the HBV whole-X gene on the expression of hepatocellular carcinoma associated proteins

Yu Zhang, Hongli Liu, Meiling Cui, Jinfeng Liu, Ruitian Yi, Yinghua Niu, Tianyan Chen, Yingren Zhao
2016 Journal of Microbiology, Immunology and Infection  
The hepatitis B virus (HBV) pre-X gene resides upstream of the HBV X gene, and together they form the HBV whole-X gene. Although it has been evident that the HBV whole-X protein is involved in the development of hepatocellular carcinoma, its biological role and molecular mechanism remain largely unknown. Methods: In this study, we subcloned the HBV whole-X gene and constructed a HBV whole-X expressing vector. After transfection of the HBV whole-X gene into HL-7702 cells, the profile of the
more » ... rential cellular protein composition in the cells was analyzed by using twodimensional electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Results: The results showed that 18 major proteins were differentially expressed in the cells transfected with or without the HBV whole-X gene. The expression of these genes was further confirmed by reverse transcription-polymerase chain reaction and Western blot analysis. Conclusion: Our findings provide a new insight into the investigation of the pathological role that the HBV whole-X gene plays in the development of hepatocellular carcinoma and may lead to the design of novel strategies for the treatment of this disease.
doi:10.1016/j.jmii.2014.07.004 pmid:25311406 fatcat:jjtvrvfv3jfw7igi42plduzram