A polysaccharide fraction was extracted from the dry delipidated chicken egg yolk by digestion with papain. Chromatography of the papain digest on Sephacryl S-300 gave a broad peak (Km, = 0.47) of dimethylmethylene blue (DMB)-reactive polysaccharide fraction, which accounted for approximately 4% of the dry-delipidated egg yolk. The fraction was not susceptible to any of the glycosaminoglycan (GAG)-degrading enzymes (e.g. Streptomyces hyaluronidase and heparitinase). It contained approximately

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... % carbohydrates consisting of galactose, mannose and glucosamine, and 39% protein with serine as the most abundant amino acid. No uronic acid was detected in the DMB-reactive fraction. It was concluded that uronic acid containing GAGs were not detected in the egg yolk.