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Synthesis and assembly of hepatitis A virus-specific proteins in BS-C-1 cells
1993
Journal of Virology
To determine the mechanism for the delayed and inefficient replication of the picornavirus hepatitis A virus in cell culture, we studied the kinetics of synthesis and assembly of virus-specific proteins by metabolic labeling of infected BS-C-1 cells with L-[35SJmethionine and L-[35Slcysteine. Sedimentation, electrophoresis, and autoradiography revealed the presence of virions, provirions, procapsids, and 14S (pentameric) subunits. Virions and provirions contained VP1, VPO, VP2, and VP3;
doi:10.1128/jvi.67.6.3095-3102.1993
fatcat:vxcpfzzmx5gcfflzacbnkrkfae