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A wide-field four-channel fluorescence imager has been developed. The instrument uses four expanded laser beams to image a large section ͑6 mmϫ 9 mm͒. An object can be sequentially illuminated with any combination of 408-, 532-, 658-, and 784-nm lasers for arbitrary ͑down to 1 ms͒ exposure times for each laser. Just two notch filters block scattered light from all four lasers. The design approach described here offers great flexibility in treatment of objects, very good sensitivity, and a widedoi:10.1117/1.3374052 pmid:20459261 fatcat:2rjawc7aefhwlildbfrpxbraz4