Estimativa da Incerteza de Medição em Análises de Benzoilecgonina em Urina por GC-MS

S. C. W. S. E. F. Oliveira, L. Y. Takai, T. F. Oliveira, M. Yonamine
2016 Brazilian Journal of Forensic Sciences, Medical Law and Bioethics  
Epidemiological studies indicate that the consumption of illegal substances is associated with a serious public health problem. To sum up, the present situation demands an immediate action, with major focus on reliable analytical methods which can be capable of detecting such compounds in biological fluids. Furthermore, the application of drug analysis in urine has increased significantly and for this reason, forensic toxicologists are being asked to determine and report their measurement
more » ... r measurement uncertainty (MU) in drug analysis. The purpose of this study was to estimate the MU of confirmatory toxicological analysis of benzoylecgonine in urine by GC-MS. The limit of detection was 5.0 ng.ml -1 . The calibration curves were linear over the specified range (7.5 -3200 ng.ml -1 ; r 2 0.99). The effect of the accuracy on the overall combined uncertainty was most significant. The developed method was successfully applied to a human urine standard reference material. The obtained relative combined uncertainty was 4.8 %. The MU obtained is in accordance with the acceptable values and are also consistent with the values stipulated by certificate of analysis of NIST reference samples. Therefore, this method is relevant and the obtained result has reliability and quality.
doi:10.17063/bjfs5(2)y2016215 fatcat:tjmyzbrecrftlncmhpy7tsvzta