Impact of DC glow discharge plasma treated Ti doped hydroxyapatite nanomaterials using antibacterial and cytotoxicity applications

P. Sri Devi, Research Scholar, Centre for Research and Evaluation, Bharathiar University, Coimbatore, Tamilnadu 641046, India, K. A. Vijayalakshmi, Sri Vasavi College, Erode, Tamilnadu 638316, India
2022 Digest Journal of Nanomaterials and Biostructures  
In this work we developed Ti doped hydroxyapatite with were synthesized by using a solgel technique with exposed to DC glow discharge plasma with different times treated. The pure and Ti/HAp were characterised by using different characterizations like XRD, FTIR, SEM, EDAX, UV-visible, PL, TG-DTA, antibacterial activity, 3T3 fibroblast cells and MCF-7 breast cancer cell line with cytotoxity efficiency analysis. The pure HAp and Ti/HAp materials had a nanocube and nanoflakee shape in the SEM
more » ... ts. In the ultraviolet-visible light (UV-vis) spectrum, the band edge absorption of bare HAp and Ti/HAp samples is discovered at wavelengths of 295 nm and 328 nm, respectively. The antibacterial activity of untreated and plasma-treated pure HAP and Ti/HAp samples are tested against two gram-positive bacteria, Bacillus cereus and Staphylococcus aureus, as well as two gram-negative bacteria, E. coli and Pseudomonas aeruginosa, using the agar well diffusion technique. The MTT assay was used to investigate the mean per cent (% percent) cell survival of Ti/HAp at various concentrations (6 mg/mL; 85 mg/mL) against 3T3 fibroblast cells and human MCF-7 Breast cancer cell lines. The synthesised Ti/HAp biocompatibility makes it a viable contender for future biological uses.
doi:10.15251/djnb.2022.174.1223 fatcat:gxi6mnxklnaztewb3djtk423qa