Evaluation of Subgingival Dental Plaque Microbiota Changes In Fixed Orthodontic Patients with Syber Green Real Time PCR

Naser Sargolzaie, Saeed Amel-Jamedar, Majid Mokhtari, Hamid Arab, Soodabe Piroozi
2014 Original Research   unpublished
One of the most common problems we confront in orthodontic therapy is periodontal diseases. Initial factor which causes these diseases is colonization of anaerobic microorganisms in subgingival plaque. Technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances. The aim of this study was to investigate changes in
more » ... estigate changes in subgingival microbiota and clinical parameters before and after bracket placement. Methods: Clinical parameters including probing depth (PD), plaque index (PI), clinical attachment level (CAL), bleeding on probing (BOP) and gingival index (GI) were recorded and subgingival microbial samples were collected in 30 people aged between 13 and 25. As a control group, 15 persons getting matched as to their age and sex with no need to orthodontic treatment were opted using specific primers, SYBER Green Real-Time PCR was carried out in order to determine bacterial flora in stored samples. All mentioned procedures were reassessed in experimental group and in control group three months after band and bracket bonding. A descriptive analysis was conducted, and paired t test and Wilcoxon test were used for differences between groups (P<0.05). Results: No changes in clinical attachment level were observed, but scores for bleeding on probing, plaque index, and gingival index increased 3 months after bracket