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The conventional sample preparation for electron microscopy makes the artifacts in the process of dehydration and substitution, such as the changes of ultrastructure of cells and outflow of soluble components in the cells. Cryofixation has been proposed as the method to overcome the inconvenient and unexpected problems, by freezing the cells very rapidly. Because water freezes to be ice crystals with increase of the volume, and those formed in the cells destroy the ultrastructure. To preservedoi:10.5685/plmorphol.25.35 fatcat:gbvw5prvrzfb7ilyg7diu3clci