Study of the Constitutively Active Form of the α Subunit of Rice Heterotrimeric G Proteins

Katsuyuki Oki, Yukiko Fujisawa, Hisaharu Kato, Yukimoto Iwasaki
2005 Plant and Cell Physiology  
We used site-directed mutagenesis to engineer two constitutively active forms of the α subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the α subunit was introduced into the rice mutant d1, which is defective for the α-subunit gene. All the transformants essentially showed a wild-type phenotype compared with normal cultivars, although seed
more » ... were substantially increased and internode lengths also showed some increase.
doi:10.1093/pcp/pci036 pmid:15695461 fatcat:tdsmdnue3vce5j4r3xotdli3mm