The Impact of SOD3 on Prostatic Diseases: Elevated SOD3 Serves as a Novel Biomarker for the Diagnosis of Chronic Nonbacterial Prostatitis [post]

Shengfeng Zheng, Hongtao Li, Xuandong Lin, Xiang Gan, Shengjun Xiao, Jiayi Liu, Zhibin Xie, Zhidi Lin, Weiru Song, Xiao Wang, Meiqing Li, Yue Lan (+3 others)
2021 unpublished
BACKGROUND: Prostate is the most common gland for the three major diseases in male, such as chronic nonbacterial prostatitis (CNP), benign prostatic hyperplasia (BPH) and prostate cancer (PCa). However, there is lack of ideal biomarker for diagnosis with prostatic diseases, especially CNP. METHODS AND RESULTS: Extracellular superoxide dismutase (SOD3) levels in serum or expressed prostatic secretion (EPS) with CNP, and in the prostate tissues of rat CNP modules, BPH, and PCa was quantified,
more » ... h showed that SOD3 was significantly increased in CNP and BPH, but decreased in PCa compared to controls. Receiver operating characteristic curve (ROC) analysis suggested that SOD3 was an efficient diagnosis biomarker discriminating CNP versus normal controls (accuracy= 0.831, 95%CI: 0.726-0.937, P<0.001), CNP III versus CNP IV (accuracy=0.868, 95%CI: 0.716-0.940, P<0.001). SOD3 were associated with the clinical characteristics of patients with CNP including pelvic pain, blood pressure, and lecithin/leukocyte in EPS. Multiple bioinformatic analysis showed that SOD3 mainly participated in superoxide radicals degradation, apoptotic execution phase, and mesenchymal-to-epithelial transition, etc. Furthermore, the structural features of SOD3 and the interacting proteins were evaluated by molecular docking, and hotspot analysis indicated that better affinities between SOD3 and its interacting molecules were associated with the presence of Arginine (Arg) in the binding site. CONCLUSIONS: According to the results, it can be concluded that SOD3 plays an important role in prostatic diseases, and it may potentially serve as an ideal diagnostic biomarker for CNP.
doi:10.21203/rs.3.rs-751353/v1 fatcat:qpwh5o36s5awngt3vx67gt3axi