Hypolipidemic Effect of the Autoclaved Extract Prepared from Pea (Pisum sativum L.) Pods In Vivo and In Vitro

Kae INAGAKI, Yuuki NISHIMURA, Emiko IWATA, Sachinobu MANABE, Masahiro GOTO, Yoshio OGURA, Hisako HOTTA
2016 Journal of Nutritional Science and Vitaminology  
By autoclaving, we obtained a polyphenol and dietary fiber from pea (Pisum sativum L.) pods in parallel without acid or alkali treatment or organic solvent extraction. Rats fed a high-sucrose (HS) diet containing 3% autoclaved extract (AE) for 4 wk exhibited significantly lower serum triglyceride and total cholesterol levels than rats fed a HS diet. AE and soluble dietary fiber (SDF) from AE exhibited pancreatic lipase inhibitory activity at 13.3 mg/mL in vitro. AE and insoluble dietary fiber
more » ... ble dietary fiber (IDF) from AE adsorbed cholesterol. In total, 30% and 10% of a cholesterol micelle were significantly adsorbed by 2,000 mg of AE and 100 mg of IDF from AE in 7 mL, respectively. The amount of bifidobacteria in the cecum of the AE group was significantly increased compared with that in the HS group. These results suggest that AE has hypolipidemic, bifidogenic potential.
doi:10.3177/jnsv.62.322 pmid:27928119 fatcat:bgrr3wt7pvcffjddfzlgfj22qa