A PREFORMULATION STUDY ON INTERACTIONS BETWEEN DOXORUBICIN HYDROCHLORIDE AND BOVINE SERUM ALBUMIN

K. C. Ajithkumar, K. Pramod
2017 International Journal of Applied Pharmaceutics  
Objective: The objective was to study the effect of concentrations of doxorubicin hydrochloride (Dox) and bovine serum albumin (BSA) in a sample on fluorescence intensity, UV absorbance, refractive index and optical rotation.Methods: A circumscribed central composite statistical design with 2 factors, 5 levels, and 13 runs was selected for the study. According to that influence of both in interaction was measured by fluorescence intensity, UV absorbance, refractive index and optical rotation
more » ... optical rotation and were analyzed by the design expert software.Results: It was observed that concentration of BSA alone was significantly affecting the fluorescence intensity and optical rotation of samples. Dox alone was having a significant effect on UV absorbance at 280 nm. In the case of a refractive index, both Dox and BSA were having a significant effect. But the effect of BSA was much pronounced than that of Dox on refractive index.Conclusion: Interaction studies between BSA and Dox would be beneficial as they are commonly used in combination with tumor-targeted delivery. The interaction was observed that in a linear model for a wide range of concentration of both. So it will be useful to determine the interaction of unknown concentration.
doi:10.22159/ijap.2017v9i6.22470 fatcat:g3eyer4jdvacjo7gk6v77eo4wa