DNA PCR Assays for Igh Rearrangement

Yusuke Satoh, Takao Sudo, Takafumi Yokota
2014 Bio-protocol  
This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013 ) [e.g. ES-derived cells (Satoh et al., 2013)].
doi:10.21769/bioprotoc.1046 fatcat:dknfum3uifcdri4s4aeas4i3lu