Tectorigenin inhibits Caco-2 human colon cells via NF-κB pathway suppression

Xiao-Yu Dai, Yong-Ming Yu, Zheng-Fang Kong, Yi-Sheng Cao, Dan-Dan Huang, Xiao-Rong Hu, Zuo-An Huang, Yang-Yang Xie, Shun Zhang
2015 Bangladesh Journal of Pharmacology  
<p class="Abstract">Effective immunomodulator and pro-inflammatory cytokine, Tumor necrosis factor-α (TNF-α) are considered to be responsible for connecting autoimmune pathological process and contagious diseases. TNF-α stimulates the C-X-C motif chemokine 10 (CXCL10) expression that is participated in migration of tumor, metastasis, and invasion. Tectorigenin, an o-methylated isoflavone, is present as a major portion in the Iris tectorum rhizomes. In this study, we investigated the
more » ... ted the tectorigenin effects as an anticancer drug. The obtained results showed that tectorigenin hinders the invasion of human colon cancer cells (Caco-2). We used reverse transcription PCR, q-PCR and enzyme linked immunosorbent assays to test whether the tectorigenin is involved in the inhibition of TNF-α induced CXCL-10 expression in the Caco-2 cell lines. Further, we tested TNF-α induced NF-κB activity using the tectorigenin. Collective results showed that tectorigenin inhibits the CXCL10 production by using TNF-α via NF-κB inhibition.</p><p> </p>
doi:10.3329/bjp.v10i4.23833 fatcat:uxkimd5hyzgxjljrgoscghah2i