INFLUENCE OF MESENCHYMAL STEM CELLS DERIVED FROM BONE MARROW OF CHILDREN WITH ONCOHEMATOLOGICAL DISEASES ON PROLIFERATION AND SELF-RENEWAL OF HEMATOPOIETIC PROGENITOR CELLS IN VITRO

Ya Isaikina, T Shman
unpublished
Aim: To test the ability of cultured mesenchymal stem cells (MSCs) from bone marrow (BM) of children with oncohematological disea­ ses after chemotherapy and radiation to support proliferation and self renewal of hematopoietic cells in vitro. Methods: BM samples of 8 patients and 9 healthy children­donors used for MSCs preparation applying techinque of expansion in vitro. CD34 + cells were isolated from BM of donors. The ability of MSCs to maintain hematopoietic stem cells (HSCs) proliferation
more » ... SCs) proliferation was tested in semisolid methylcellulose medium and in liquid long­term­culture (LTC) medium. Results: The presence of MSCs derived from BM of patients in methylcellulose medium induced 2­fold increase of the number of commited myeloid progenitors without cytokines, 7­fold increase together with growth factors and 14­fold increase of the amount of earlier pluripotent hematopoietic precursor cells (CFU­GEMM) compared to expansion of HSCs without MSCs and cytokines. The presence of MSCs layer of patients in liquid LTC medium sig­ nificantly promoted the hematopoietic cells proliferation rate, measured on 7th, 14th and 21st day. The total number of cells was multiplied 161.2­fold on 21st day as compared to 116.4­fold without MSCs layer. In the presence of MSCs layer, we detected the increase of proportion of bipotent CFU­GM precursors from 4% to 11% and pluripotent CFU­GEMM precursors from 0.1% to 0.6% in population of HSCs. In both types of experiments the capacity of patients' MSCs to support HSCs proliferation and self renewal was the same as for healthy donors' MSCs. Conclusion: In this study, МSCs were isolated from BM of children with malignancies after high­dose chemotherapy or radiation. The ability of these MSCs to maintain hematopoiesis in vitro was tested. It was shown that co­transplantation of autologous MSCs is a good way to improve hematopoietic stem cells engraftment and reduce a period of granulocytopenia after autologous HSCs transplantation in case of insufficient CD34 + cell number in autologous transplant.
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