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Purification and characterization of cytosolic and cell wall β-galactosidases from Vigna unguiculata stems
Brazilian Journal of Plant Physiology
Three β-galactosidase isoforms, β-gal I and β-gal II (cytosolic) and β-gal III (cell wall-associated), were isolated from stems of Vigna unguiculata (L.) Walp. cv. Pitiúba seedlings. Purification consisted of ammonia sulfate fractionation followed by chromatography in DEAE-Sephadex and Lactosyl-Sepharose columns. The two cytosolic isoforms showed the same chromatography pattern, which differed from that of β-gal III. Electrophoresis revealed a single band of protein for β-gal II and β-gal IIIdoi:10.1590/s1677-04202011000100003 fatcat:sqn4l6lffjbd7mdsppfndhrr7m