GENETIC MARKER ASSISTED SELECTION FOR COMMON BUNT RESISTANCE (TILLETIA SP.) IN SOME WHEAT LINES

Laura Cristina Cota, C. Botez, Dana Bota, Meda Lucaci
1970 Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Agriculture  
In view of the molecular marker assisted selection for common bunt resistance, wheat lines in segregation have been used, as well as stabilized dihaploid wheat lines – resulted from different hybrid combinations in which parental forms resistant to bunt were used. To identify the polymorphisms between the resistant and sensitive forms, a pair of specific primers for the common bunt resistant gene (Bt-10) were used, as well as a number of twenty non-specific RAPD (Random Amplified Polymorphic
more » ... ) primers. The main objective was finding some polymorphic markers for common bunt resistance in view of the selection of these lines on the basis of the markers. Such a marker were obtained (1450 bp) with specific primers, a marker linked to Bt-10 gene, and with non-specific primers more polymorphic fragments were obtained, present in the resistant forms and absent in the sensitive forms, possibly linked to common bunt resistant genes. In perspective, it is stipulated the conversion of these RAPD molecular markers to SCAR (Sequence Characterized Amplified Region) specific markers.
doi:10.15835/buasvmcn-agr:1348 fatcat:o77l22cygnb3dnda3k7kq4vjpq