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Gene dosage screens in yeast reveal core signalling pathways controlling heat adaptation
Heat stress causes proteins to unfold and lose their function, jeopardizing essential cellular processes. To protect against heat and proteotoxic stress, cells mount a dedicated stress-protective programme, the so-called heat shock response (HSR). Our understanding of the mechanisms that regulate the HSR and their contributions to heat resistance and growth is incomplete. Here we employ CRISPRi/a to down- or upregulate protein kinases and transcription factors in S. cerevisiae. We measure genedoi:10.1101/2020.08.26.267674 fatcat:ajbdkun3hncqpoo3ainlr4qzni