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M odares Journal of M edical Sciences: Pathobiology
Objective: This study evaluates the effect of a mouse fallopian tube cell co-culture on the proliferation of human endometrial stromal cells. Methods: We used hysteroscopy to collect the endometrial cells from the uterus. The cells were isolated with collagenase type 3 and passed through 150 µm and 40 µm filters, respectively, after which the isolated cells were cultured in DMEM/F12 medium. At the end of the fourth subculture we used flow cytometry to evaluate the percentage of CD90 positivefatcat:7lj7ap73ovdnjnjeqdieacakty