ABSTRACT. The chemical composition of hydro-distilled essential oil of dried roots of Anaphalis contorta Hook F. was analyzed for the first time by using gas chromatography equipped with flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). The compounds of essential oil were identified according to their mass spectra and their relative retention indices determined on a capillary GC column (non-polar stationary phase). Twenty-seven compounds were

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... ified representing 94.8% of the total oil. The major constituents were 2,2-dimethyl-2[2,4,6-trimethylphenyl] acetic acid (12.1%), labda-8,14-dien-13-ol (8.4%), δ-cadinene (8.1%), labda-7,14-dien-13-ol (6.9%), α-gurjunene (6.7%), viridiflorene (5.1%), and caryophyllene oxide (4.9%). The root essential oil of A. contorta produced different chemotypes. KEY WORDS: Anaphalis contorta Hook F., Essential oil composition, 2,2-Dimethyl-2[2,4,6-trimethylphenyl] acetic acid. labda-8,14-dien-13-ol, GC/MS Bull. Chem. Soc. Ethiop. 2022, 36(1), 235-240. DOI: https://dx.doi.org/10.4314/bcse.v36i1.19