Standardization of Protocol for in vitroMicropropagation of Phyllanthus niruri: An Important Medicinal Plant
UK Journal of Pharmaceutical Biosciences
An efficient regeneration protocol for Phyllanthus niruri was developed using nodal explants on MS medium augmented with different concentrations and combinations of plant growth regulators. The highest frequency (85.5%) of bud break and shoot induction was observed on medium supplemented with 1.0 mg/l BAP along with 0.5 mg/l IBA with the formation of 4.2±0.5 number of shoots having length of 3.0±0.1 centimeters within ten days. MS medium fortified with 1.0 mg/l BAP and 1.0 mg/l Kinetin in
... g/l Kinetin in combination with NAA (0.5 mg/L) showed the highest percentage (80.0%) shoot multiplication with an average of 9.0±0.7 adventitious shoots, directly from the explants, without any callus formation. Shoots formed were remarkable healthy and achieved the length of 10.0±0.5 centimeters in 25 days. Elongated shoots were rooted best on MS medium with 1.0 mg/l IBA producing maximum number of roots with 85% response with average length 8.0±0.3 cm within 15 days. The plantlets were gradually acclimatized and successfully transferred to field condition with 98% survival rate after rooting. The standardized protocol reported in this study may help in large scale propagation of this plant species which is currently exploited from the nature.