Optimization of fluorophore-assisted carbohydrate electrophoresis

Gang L Huang
Zeitschrift fur Naturforschung. Section C, Biosciences  
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating saccharides. Oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and the reductive amination reactions were essentially complete after approximately 16 h under the given experimental conditions. Saccharide-ANTS adducts were then separated by electrophoresis on 32% C(ACR)/2.4% C(BIS) polyacrylamide gel at alkaline pH. This technique
more » ... is technique doesn't require sophisticated instrumentation and highly trained personnel.
pmid:18811010 fatcat:guffxgp3irbmhomqv2pziqvv2m