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Nitric Oxide Modulates Low-Mg2+-Induced Epileptiform Activity in Rat Hippocampal–Entorhinal Cortex Slices
2002
Neurobiology of Disease
The production of nitric oxide (NO) during low-Mg 2؉ -induced epileptiform activity in rat hippocampalentorhinal cortex slices was investigated by real-time monitoring using 1,2-diaminoanthraquinone (DAQ). NO reacts with the aromatic amino groups of DAQ at neutral pH and in the presence of oxygen to form the fluorescence product 1H-anthra-[1,2d]-[1,2,3]triazole-6,11-dione (ATD). The DAQ-induced formation of ATD required NO and was insensitive to radical oxygen species. Removal of Mg 2؉ ions
doi:10.1006/nbdi.2002.0533
pmid:12460549
fatcat:4herk7szmrf73ozacbb27xvi4q