Waste biomass and agro-industrial by-products, for production ethanol, will meet much of the great demand for this product. To reduce costs and optimize production, this study investigated solid-state fermentation (SSF) to obtain crude enzyme complex (CEC) from different agro-industrial biomasses (sugarcane bagasse, corn peel bran, rice straw bran and roasting and ground coffee residue) using cellulolytic fungi. The most promising CEC were evaluated in simultaneous hydrolysis and fermentation

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... HF) for ethanol production by Saccharomyces cerevisiae in a culture broth containing sugarcane bagasse treated by steam explosion, and roast and ground coffee residue. In SSF with bioreactor volume of 0.25 L, containing 40 g of the biomass mixture and 40 g of sterile water with resuspended cells (1.0 x108 spores/g of solid medium) and temperature of 30±2 ºC, the strains Trichoderma reesei and Penicilium oxalicum provided the best enzyme activity. The CEC of T. reesei provided a concentration of 7.5 g L-1 of ethanol in a substrate containing treated sugarcane bagasse (60%) and roast and ground coffee residue (40%), under SHF conditions (pH 4.5, 35±2 °C, 48 h). The results obtained in this study show a promising alternative for correct disposal and use of residues and agro-industrial by-products by use in the production of enzymes and lignocellulosic ethanol.