GENE EXPRESSION ANALYSIS OF OLIVE TREE (OLEA EUROPAEA L.) IN RESPONSE TO SALT STRESS

C. Bazakos, M. Manioudaki, I. Therios, D. Voyiatzis, D. Kafetzopoulos, E. Sarropoulou, T. Awada, P. Kalaitzis
2011 Acta Horticulturae  
Olive is an ever-green tree of the oleaceae family and of the genus olea , grown under a wide range of climates and soils all over the world. In Iran, 50 percent of all growing areas encountered by high salt concentration conditions and drought. Water deficit caused by drought and high salinity has been an important factor limiting crop productivity. To cope with these environmental stresses, application of molecular plant breeding is necessary. In response to osmotic stress elicited by
more » ... n of high salt, the expression of P5CS gene increased in olive plants. Transgenic and non transgenic olive plants were transferred to the solid ½ MS containing 200 mM NaCl and they were sub-cultured about 2 weeks, then total protein was extracted from fresh leaves of transgenic plants and non transgenic plants under osmotic stress condition and measured using of Bradford to subject to SDS-PAGE gel electrophoresis. To probe the effect of P5CS overexpression on salinity stress tolerance, polyclonal antibody developed in rabbit against P5CS protein was used. The result of immunological methods for specific detection of P5CS gene, strongly indicated P5CS up -regulation in stressed transgenic olive plants versus stressed non transgenic olive plants.
doi:10.17660/actahortic.2011.924.4 fatcat:o2jaltzsjvbm5oeyglygnikthe