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Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis
2021
Biosensors & bioelectronics
Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR or sequencing are tough to implement with limited resources, necessitating the development of accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout for
doi:10.1016/j.bios.2021.113207
pmid:33866136
pmcid:PMC8020606
fatcat:6g7z3zrya5g5znxwpn6j7mlfwe