Mercury resistance as a selective marker for recombinant mycobacteria

A. Baulard, V. Escuyer, N. Haddad, L. Kremer, C. Locht, P. Berche
1995 Microbiology  
The use of antibiotic-resistance markers for the selection of recombinant mycobacteria is widespread but questionable considering the development of live recombinant BCG vaccines. In contrast, vector-encoded resistance to heavy metals such as mercury may represent an interesting alternative for the development of live vaccines compatible with use in humans and in animals. The mercury resistance genes (mer) from Pseudomonas aemginosa and from Serratia marcescens were cloned into the Escherichia
more » ... to the Escherichia coli-Mycobacterium shuttle vector pRR3. The resulting vectors, designated pMROOl and pVN2, were introduced by elect ro pora t ion in to Mycobacterium smegma tis, Mycobacterium bowis BCG and Mycobacterium tuberculosis. The recombinant mycobacteria were stable in witro and in wiwo, and had high-level mercury resistance, thus indicating that the mer genes can be useful as selective markers in mycobacteria. Abbreviations: BCG, M. bovis bacille CalmetteGuerin; PMA, phenylmercuric acetate. recent years, rendered mycobacteria amenable to molecular investigations.
doi:10.1099/13500872-141-4-1045 pmid:7773382 fatcat:ddqjqzsywrfinlb7mwdnqiesau