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The use of antibiotic-resistance markers for the selection of recombinant mycobacteria is widespread but questionable considering the development of live recombinant BCG vaccines. In contrast, vector-encoded resistance to heavy metals such as mercury may represent an interesting alternative for the development of live vaccines compatible with use in humans and in animals. The mercury resistance genes (mer) from Pseudomonas aemginosa and from Serratia marcescens were cloned into the Escherichiadoi:10.1099/13500872-141-4-1045 pmid:7773382 fatcat:ddqjqzsywrfinlb7mwdnqiesau