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Extracellular polymeric substances (EPS) are pivotal in the protection of biofilm inhabitants against mechanical and chemical challenges. EPS production in a Staphylococcus aureus biofilm stimulated by fluid shear and mechanical pressure can be studied using a TriboChemist. The TriboChemist is a recently introduced instrument, allowing the study of initially adhering bacteria to a germanium crystal using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, whiledoi:10.1128/aem.02420-17 fatcat:ildju6whu5bnhicvydnf34rlt4