Synergistic effect of BMP9 and TGF-β in the proliferation and differentiation of osteoblasts

X.L. Li, Y.B. Liu, E.G. Ma, W.X. Shen, H. Li, Y.N. Zhang
2015 Genetics and Molecular Research  
We investigated the synergistic effect of bone morphogenetic protein 9 (BMP9) and transforming growth factor (TGF)-b in the transformation of mesenchymal stem cells into osteoblasts. We evaluated the effect of BMP9 and TGF-b on the induction of osteoblast formation. Mitogen-activated protein kinase (MAPK) pathway-related proteins such as p38, extracellular receptor kinase 1/2, and c-Jun N-terminal kinase (JNK) were analyzed. The interactions between TGF-Smad and BMP-MAPK were also studied. BMP9
more » ... also studied. BMP9 alone induced the differentiation of mesenchymal stem cells (MSCs) into osteoblasts and enhanced phosphorylation of p38, extracellular receptor kinase 1/2, and JNK. TGF-b alone failed to induce transformation, but could increase the effect of ©FUNPEC-RP Genetics and Molecular Research 14 (3) : 7605-7615 (2015) BMP9. In this process the activation of Smad resulted in activation of the JNK pathway in the MAPK pathway. BMP9 induced osteogenesis of MSC differentiation through the MAKP pathway, while TGF-b contributed to BMP9 enhancement through the Smad-JNK pathway.
doi:10.4238/2015.july.13.4 pmid:26214439 fatcat:ocypfrxhrvdutiw3nvr3h72n3m