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Validation of Ultra High Pressure Liquid Chromatography (UHPLC) with Pre-column Derivatization Method for Quantitative Analysis of Histamine in Fish and Fishery Products
2019
The Philippine Journal of Fisheries
A B S T R A C T A pre-column derivatization-ultra high performance liquid chromatographic (UHPLC) method is described for the determination of histamine in fish and fishery products. The homogenized samples were extracted with trichloroacetic acid (TCA) solution and derivatized with o-phthaldialdehyde. Histamine was separated using reversed-phase column and determined using UHPLC with fluorescence detection. The linear calibration range was 10 to 60 µg/mL with a correlation coefficient of
doi:10.31398/tpjf/26.2.2018a0004
fatcat:rxkvszbubjhjhl35k5xburza5m