Guidelines for Adapting Light Microscopy Deconvolution Methods to 3D Biological STEM

B Northan, R Sougrat, N de Jonge
2009 Microscopy and Microanalysis  
Advances in spherical aberration correction of the scanning transmission electron microscope (STEM) have led to a reduced depth of field of the STEM probe [1]. This has made it possible to form a 3D dataset by the stacking of 2D images collected at a sequence of depth intervals through the sample thickness. Each 2D image contains the signal from objects at its corresponding depth within the sample and also the out-of-focus signals from the adjacent images (above and below). A similar effect
more » ... rs in widefield fluorescence light microscopy, where the effect is corrected for by applying a deconvolution algorithm, of which several variants exist. We discuss modification of the Expectation-Maximization deconvolution procedure originally developed for light microscopy [2], and its subsequent application to 3D STEM.
doi:10.1017/s1431927609093532 fatcat:yldorvm3dffrxa67s5hmmtfplq