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Guidelines for Adapting Light Microscopy Deconvolution Methods to 3D Biological STEM
2009
Microscopy and Microanalysis
Advances in spherical aberration correction of the scanning transmission electron microscope (STEM) have led to a reduced depth of field of the STEM probe [1]. This has made it possible to form a 3D dataset by the stacking of 2D images collected at a sequence of depth intervals through the sample thickness. Each 2D image contains the signal from objects at its corresponding depth within the sample and also the out-of-focus signals from the adjacent images (above and below). A similar effect
doi:10.1017/s1431927609093532
fatcat:yldorvm3dffrxa67s5hmmtfplq