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Rapid Detection of Flt3 Mutations in Acute Myeloid Leukemia Patients by Denaturing HPLC
fms-related tyrosine kinase 3 (Flt3) is the most commonly mutated gene in human acute myeloid leukemia (AML) and has been implicated in its pathogenesis. Because screening of Flt3 in AML patients by PCR followed by gel electrophoresis is time-consuming and fails to detect some very small internal tandem duplications (ITDs), we developed a method for screening of FLT3 receptor mutations with PCR plus denaturing HPLC (D-HPLC). Methods: Total mRNAs extracted from 34 AML patients were firstdoi:10.1373/49.10.1642 pmid:14500589 fatcat:tvz743s4yzf2rm4wcez3wqzexe