Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay

Seyedeh Mahya Marashiyan, Fatemeh Moradian, Geita Saadatnia, Majid Golkar
2016 Archives of Clinical Infectious Diseases  
Infections by the protozoan parasite Toxoplasma gondii (T. gondii) are widely prevalent in human and animals. SRS3 is a member of SRS antigen family (glycosylphosphatidylinositol-linked proteins), which is structurally related to the highly immunogenic surface antigen SAG1. The SAG family of proteins can cover the surface of T. gondii bradyzoites and tachyzoites. Objectives: The aim of this study was to evaluate the recombinant SRS3 protein for diagnosis of toxoplasmosis by enzyme-linked
more » ... orbent assay. Methods: Indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) with recombinant SRS3 and SAG1 proteins of T. gondii were developed to evaluate the diagnosis of infection in pregnant women. Results: The results showed that sensitivity and specificity were respectively 84.12% and 92% for recombinant protein SRS3 and 92% and 96% for rSAG1. Conclusions: Recombinant proteins SRS3 and rSAG1 are promising antigens in diagnostic assays for detection of specific antibodies against T. gondii.
doi:10.5812/archcid.35612 fatcat:j6kmpzgzjvccjlfvs6z7ay5knu