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IDENTIFICATION OF A MIR-212 TARGET SITE IN KIR2.1 3′ UTR BY A NOVEL DUAL-FLUORESCENCE MICRORNA TARGETING ASSAY
2012
Heart
MicroRNAs (miRNAs) are increasingly recognised as important down-regulators of gene expression in health and disease. Luciferase-based assays are commonly used for identifying miRNA targets but these have limitations. We report miRNA target identification using a novel functional miRNA targeting assay. The 3 0 UTR of the human inward rectifier K+ channel Kir2.1 was inserted downstream of the mCherry red fluorescent protein sequence in a mammalian expression plasmid. MiRNA or non-targeting
doi:10.1136/heartjnl-2012-303148a.15
fatcat:nvpoadjaibeb3kr2ta2lxqezc4