Lipid Dynamics due to Muscle Atrophy Induced by Immobilization

Keisuke Kimura, Mizuki Morisasa, Takafumi Mizushige, Rikuo Karasawa, Chinatsu Kanamaru, Yukihito Kabuyama, Takahiro Hayasaka, Tsukasa Mori, Naoko Goto-Inoue
2021 Journal of Oleo Science  
Muscle atrophy refers to skeletal muscle loss and dysfunction that affects glucose and lipid metabolism. Moreover, muscle atrophy is manifested in cancer, diabetes, and obesity. In this study, we focused on lipid metabolism during muscle atrophy. We observed that the gastrocnemius muscle was associated with significant atrophy with 8 days of immobilization of hind limb joints and that muscle atrophy occurred regardless of the muscle fiber type. Further, we performed lipid analyses using thin
more » ... er chromatography, liquid chromatography-mass spectrometry, and mass spectrometry imaging. Total amounts of triacylglycerol, phosphatidylserine, and sphingomyelin were found to be increased in the immobilized muscle. Additionally, we found that specific molecular species of phosphatidylserine, phosphatidylcholine, and sphingomyelin were increased by immobilization. Furthermore, the expression of adipose triglyceride lipase and the activity of cyclooxygenase-2 were significantly reduced by atrophy. From these results, it was revealed that lipid accumulation and metabolic changes in specific fatty acids occur during disuse muscle atrophy. The present study holds implications in validating preventive treatment strategies for muscle atrophy.
doi:10.5650/jos.ess21045 pmid:34193670 fatcat:xfawxpgssvffxiik4vv5tiajri