Do TMS evoked responses measured from scalp and hand represent the same cortical mechanisms? [article]

Mana Biabani, Alex Fornito, James P. Coxon, Ben D. Fulcher, Nigel C. Rogasch
2019 bioRxiv   pre-print
Transcranial magnetic stimulation (TMS) is a powerful tool to investigate local cortical circuits and broader neural networks. Paired-pulse TMS (ppTMS) paradigms are commonly employed to study excitatory/inhibitory neurotransmissions in motor circuits across time by assessing changes in motor-evoked potentials (MEPs) using electromyography (EMG). The combination of TMS with electroencephalography (EEG) has the capacity to extend this work outside the motor system by focusing on TMS-evoked
more » ... on TMS-evoked potentials (TEPs) across both space and time as the measurable output of brain stimulation. However, the relationship between these two putative outputs of TMS effects - MEPs and TEPs - remains unclear. Aim: To investigate whether the same neural populations are responsible for fluctuations in MEPs with ppTMS as the TEP waveform following single pulse TMS (spTMS). Methods: Twenty-four healthy participants received intra-hemispheric and interhemispheric (dual-coil) ppTMS, with different inter-pulse intervals, conditioning intensities, and TMS pulse waveforms over the motor cortex, while EMG was recorded from first dorsal interosseous muscles. EEG was also recorded in response to spTMS with the same intensity and location as the conditioning pulses in ppTMS-EMG. Additionally, TMS was applied to the shoulder as a multisensory control condition. The relationship between ppTMS-EMG and spTMS-EEG were evaluated using metrics sensitive to both the shape and amplitude of the signals. Results: The fluctuations in cortical excitability following suprathreshold TMS were correlated in shape, but not amplitude, when measured with ppTMS-EMG and TMS-EEG. This relationship was observable only after reversing the polarity of short latency TEPs (<~60 ms). For interhemispheric measures, supressing sensory potentials in TEPs was required to discern the relationship between the two signals. Conclusion: The relationship between MEP and TEP measures suggests both signals reflect activity from overlapping neural populations. This finding establishes a fundamental link between TEP peaks and periods of net excitation/inhibition observed with ppTMS-EMG in both the stimulated motor cortex and connected cortical regions.
doi:10.1101/765875 fatcat:bkrtjcq47rahpnz6fqe2g5m66i