Calorific contents of dried cells of several representative species of bacteria (gramnegative rods and gram-positive rods and cocci), two species of yeasts, and a filamentous fungus were determined by bomb calorimetry. The grand mean was 5,383 cal per g of ash-free dry weight. This value was then used to determine quantity of energy assimilated (E.) during growth. Subsequently, E. was employed in the equation: Ykcal = Y/(Es + Ed), where Ykcal is the yield of cells per kilocalorie of energy

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... from a culture medium, Y is the yield per mole of substrate utilized, E. is Y times caloric content of the cells, and Ed is the energy expended by oxidative