Metabolomics is an essential tool for not only understanding the pathophysiology of various diseases but also for searching for initial clues to unknown toxic effects of drugs. Mass spectrometry-based metabolomics has achieved highly sensitive and selective analysis of metabolites, and gas chromatography mass spectrometry remains a gold standard because of its robustness and usability. However, it is tedious to annotate metabolites with electron ionization (EI)-based mass spectra; thus, gas

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... matography tandem mass spectrometry (GC/MS/MS)-based metabolome analysis has played an important role in metabolomics. In particular, the selected reaction monitoring (SRM) mode achieves higher selectivity and an improved signal-to-noise ratio, which leads to easier metabolite identification. In this mini review, we concisely outline the pros and cons of GC/MS/MS-based metabolome analysis and provide its applications to pathophysiological analysis of disease and drug-induced toxicity in animal models based on our previous studies. Finally, future perspectives for newly developed high-throughput metabolome analysis are briefly described.