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This paper evaluates the cleaning efficiency of glycolipid-based anionic biosurfactant produced by a local company. Before practical application in removal of oil or other hydrophobic substances from soil, it is necessary to learn the behavior of the biosurfactant across different systems. As process variables, temperature of environment, contact time with dilution of biosurfactant, and concentration of biosurfactant in washing solution were modeled by applying full factorial design. Asdoi:10.7763/jocet.2014.v2.90 fatcat:vs26nk7qvfg6hasymv3bps2vlq