Some cases of sudden deaths in cattle, sheep and goats reported to the National Veterinary Laboratory, Gaborone were investigated. The clinical signs included: stupor, weakness, staggering, trembling, dyspnoea (difficulty in breathing), salivation, diarrhoea, and convulsions for those in extremis. The most consistent sign was the muddy coloured mucous membranes. Necropsy findings included: marked gastroenteritis and generalized congestion and petichiation in all internal body organs and

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... e brown coloured blood that clotted poorly. The presence of nitrate/nitrite in the blood of live animals, amniotic fluid, urine, ocular fluid, urine, drinking water and consumed forage were demonstrated by the diphenylamine test and subsequently confirmed by Gries test. Further confirmation of nitrate/ nitrite poisoning was by response to therapeutic intervention with methylene blue using the intravenous route of inoculation. Environmental factors that may have contributed to the poisoning episodes included, drought, high ambient temperatures, low cloud cover, soil leaching after flooding, soil moisture content and forage nitrogen content and natural geoformations associated with high nitrate content in the water. This is the first account of confirmed cases of nitrate/ nitrite poisoning in livestock in Botswana.