A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is
Development of a Shuttle Vector for Nocardioforms
Acta Agriculturae Serbica
Construction of Escherichia coli-nocardioforms shuttle vector pAR1 was achieved using the recombinant DNA technology. Developmental strategy included following cloning and fusion steps. Firstly, insertion of kanamycin resistance cassette into the suicide vector pEcoR251 was performed. This was followed by ligations of a nocardial replicon from the pCY104 and EcoRI endonuclease from the pEcoR251, lacking ampicillin resistant determinant. New recombinant plasmid was engineered with a positivefatcat:5e5bv5wygngavgeaumz2jjyntq